Vol. 28, Nº e

Abordagem no ensino da dissecação do diafragma em primatas não-humanos

Lessa, T.B.; Silva, L.C.S.; Constantino, M.V.P.; Santos, P.R.S.; C.E. Ambrósio; P.P. Bombonato; A.C. Assis Neto

ABSTRACT: Objectives: To address the dissection method of the diaphragm muscle in nonhuman primates, refining the technique of dissection of structures in the transverse axis. Methods and Results: Were used four adult animals of specie Callithrix jacchus (white-tufted-ear-marmoset) and four animals of Callitrix geoffroyi (Black-tufted-ear-marmoset), two males and two females of each species. All of it was from a breeding of wild animals, located in Atibaia/SP and eventually died of natural causes. These animals, after receipt at the Laboratory of Anatomy of Domestic and Wild Animals, College of Veterinary Medicine and Animal Science of Sao Paulo (FMVZ-USP) were fresh dissected, following shaving the chest and abdome, using the materials: scissors, tweezers, bistoury and slide. Primates analyzed were positioned in dorsal position and the thoracic and pelvic limbs were fixed with a bandage for better handling during dissection. Subsequently were proceeded an incision in the median sagittal plane and bounce towards the dorsal skin of the sternum manubrium to the umbilical scar, followed by removal of the subcutaneous fascia and antimeres muscle group s, allowing access to the diaphragm muscle, both by its thoracic or abdominal face. Thereafter were proceeded its removal, carefully, by an incision along the costal, sternal and lumbar portions or pillars. We observed three openings in its extension of the caudal vena cava foramen, aortic hiatus and esophageal hiatus. Still was possible to observe between the pericardium and diaphragm the phrenicpericardial ligament, the costal and sternal faces and the diaphragm (Figure A and B). Conclusions: We concluded that the dissection technique on the diaphragm in the Callithrix jacchus and C. geoffroyi species was very effective. Preserving the other organs which were located at thorax and abdome. Also enable the visualization of structures inserted on the transverse axis. Therefore this technique could be projected to other species of nonhuman primates.